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Image Search Results
Journal: Nature Communications
Article Title: A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure
doi: 10.1038/s41467-017-00171-w
Figure Lengend Snippet: Mechanism for AGGF1 regulation of miR-183-5p expression. a ChIP–qPCR analysis for the interaction between ZEB1 and miR-183-5p promoter DNA ( n = 4/group, ** P < 0.01). b Schematic diagram showing that the miR-183-5p promoter-luciferase reporter with two ZEB1-binding motifs. c Luciferase activity ( n = 6/group, ** P < 0.01). d Real-time RT-PCR analysis for miR-183-5p expression in H9C2 cells after AGGF1 treatment with or without ZEB1 overexpression ( n = 3/group, ** P < 0.01). e Western blot analysis for the ZEB1 level in H9C2 cells after AGGF1 treatment ( n = 5/group, * P < 0.05). f Western blot analysis for activation of ERK1/2 in H9C2 cells after AGGF1 treatment ( n = 5/group, ** P < 0.01). g Real-time RT-PCR analysis for ERK1 and ERK2 expression by ERK siRNA ( n = 3/group, ** P < 0.01). h Western blot analysis for the ZEB1 level by ERK siRNA ( n = 5/group, * P < 0.05). i Schematic model for a non-canonical ER stress signaling pathway mediated by AGGF1. Data are shown as the mean ± s.d. from at least three independent experiments. Statistical analysis was carried out by a Student’s two-tailed t -test
Article Snippet: Protein–DNA complexes were immunoprecipitated with IgG or an
Techniques: Expressing, ChIP-qPCR, Luciferase, Binding Assay, Activity Assay, Quantitative RT-PCR, Over Expression, Western Blot, Activation Assay, Two Tailed Test